In the video, which was taken on February 16, Game is seen standing with his entourage in the VIP section of the Bourbon Bar nightclub, where a group of partygoers attempt to get his attention.
Seemingly unprovoked, he 32-year-old rapper then clearly leans forward, fuming, and spits at the group of girls, one of whom shouts "how dare you?" angrily, in response.
Brydie McCreadie, 19, who sent her video of the incident to Australia's ninemsn, said that she was "disgusted" when realized she had just been spit on.
"I was like, 'whoa'. At first I wasn't sure if he was spitting at me," she told ninesm. "He was sitting there drinking. We were all a bit drunk. I think they were just excited to see him."
In the video, Game is then seen handing off his diamond-encrusted chain to one of his entourage members, apparently about to jump into a brawl with the group of girls.
"I think he was about to start a fight or look tough," McCreadie concluded.
Thankfully, the disappointed fans left the club before the situation escalated. Game has a history of such incidents, recently confronting a fan while on tour in Oslo, assaulting a man during a basketball game, and punching a man outside of the record release party for his most recent LP, 'The R.E.D. Album.'
Watch Game Spit on Fans